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Carbon dots-silver nanoparticles fluorescence resonance energy transfer system as a novel turn-on fluorescent probe for selective determination of cysteine

Paper ID Volume ID Publish Year Pages File Format Full-Text
26625 43968 2015 7 PDF Available
Title
Carbon dots-silver nanoparticles fluorescence resonance energy transfer system as a novel turn-on fluorescent probe for selective determination of cysteine
Abstract

•Interaction of carbon dots with AgNPs was investigated.•The fluorescence of C-dots is efficiently quenched by AgNPs.•C-dot-AgNP FRET system was used as a selective turn-on sensor for cysteine.•Using this system the LOD of 6.0 nM was obtained for cysteine.

In this paper, we investigated the interaction of carbon dots (C-dots) prepared from orange juice with silver nanoparticles (AgNPs) using fluorescence spectroscopy. It was found that AgNPs efficiently quench the fluorescence of C-dots as a result of fluorescence resonance energy transfer (FRET). Thus, a novel FRET system between C-dots (as the donor) and AgNPs (as the acceptor) was introduced. Moreover, it was found that cysteine even at nanomolar levels could recover the fluorescence of C-dots due to the competitive adsorption of this compound onto AgNPs. This was exploited to design a simple and selective method for the determination of cysteine in the concentration range from 6.0 to 300 nM, with a detection limit of 4.0 nM. Cysteine was determined by this method in human plasma and urine samples with satisfactory results.

Keywords
Carbon dots; Silver nanoparticles; Fluorescence resonance energy transfer; Cysteine
First Page Preview
Carbon dots-silver nanoparticles fluorescence resonance energy transfer system as a novel turn-on fluorescent probe for selective determination of cysteine
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 309, 15 August 2015, Pages 8–14
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering