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Near ultraviolet radiation-mediated reaction between N-nitrosoproline and DNA: Isolation and identification of two new adducts, (R)- and (S)-8-(2-pyrrolidyl)-2′-deoxyguanosine

Paper ID Volume ID Publish Year Pages File Format Full-Text
26686 43971 2014 7 PDF Available
Title
Near ultraviolet radiation-mediated reaction between N-nitrosoproline and DNA: Isolation and identification of two new adducts, (R)- and (S)-8-(2-pyrrolidyl)-2′-deoxyguanosine
Abstract

•UVA-mediated reaction between N-nitrosoproline and DNA.•Identification of five possible guanine modifications.•Three of already known and two not described photoproducts were identified.

To explore possible genotoxicity of N-nitrosoproline (NPRO), we investigated near-ultraviolet radiation (UVA)-mediated chemical reaction of NPRO with 2′-deoxyguanosine (dG). An acidic solution containing NPRO and dG was irradiated with UVA and products were analyzed by HPLC. In addition to the three known modified guanine nucleosides, i.e., 2′-deoxyxanthosine, 8-oxo-7,8-dihydro-2′-deoxyguanosine and 2′-deoxyoxanosine, we found two products. These new products were isolated and identified as (R)- and (S)-8-(2-pyrrolidyl)-2′-deoxyguanosine (G1 and G2, respectively). Experiments using monochromatic UVA in the range 300–400 nm were performed to determine the relation between products-yield and wavelength, and the highest yields of both G1 and G2 were found to occur at 340 nm. This wavelength coincided with the absorption maximum of NPRO. We propose that the NO radical produced from NPRO by photolysis triggers the formation of these products. Further investigation using calf thymus DNA showed that G1 and/or G2, probably both, could be produced by UVA irradiation of DNA + NPRO as well.

Keywords
NPRO, N-nitrosoproline; UVA, near-ultraviolet light; dG, 2′-deoxyguanosine; NO, nitric oxide; 8-oxodG, 8-oxo-7,8-dihydro-2′-deoxyguanosine; dO, 2′-deoxyoxanosine; dX, 2′-deoxyxanthosinePhotoreaction; N-nitrosoproline; DNA adducts; 8-Oxo-deoxyguanosine; NO
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Near ultraviolet radiation-mediated reaction between N-nitrosoproline and DNA: Isolation and identification of two new adducts, (R)- and (S)-8-(2-pyrrolidyl)-2′-deoxyguanosine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 276, 15 February 2014, Pages 1–7
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us