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Synthesis, characterization, DNA binding and cleavage, HSA interaction and cytotoxicity of a new copper(II) complex derived from 2-(2′-pyridyl)benzothiazole and glycylglycine

Paper ID Volume ID Publish Year Pages File Format Full-Text
26695 43971 2014 13 PDF Available
Title
Synthesis, characterization, DNA binding and cleavage, HSA interaction and cytotoxicity of a new copper(II) complex derived from 2-(2′-pyridyl)benzothiazole and glycylglycine
Abstract

•Noncovalent interactions of a new Cu(II) complex with DNA and HSA were studied.•The complex exhibited considerable DNA-binding and nuclease activity.•The complex showed desired affinity to HSA via hydrophobic interaction.•MTT assay revealed prominent and selective cytotoxicity toward HepG2.

A new copper(II) complex, [Cu(glygly)(pbt)(H2O)]ClO4 (glygly = glycylglycine anion and pbt = 2-(2′-pyridyl)benzothiazole) was synthesized and characterized by elemental analysis, molar conductivity, mass spectra, IR spectra, UV–vis spectra and thermogravimetric analysis (TGA). Spectroscopic titration, viscosity, and electrophoresis measurements revealed that the complex intercalated to calf thymus (CT)-DNA with moderate binding affinity (Kb = 5.64 × 104 M−1), and cleaved pBR322 DNA at a low concentration of 5 μM in the presence of ascorbic acid, presumably via an oxidative mechanism. Further, the protein-binding ability has been monitored by various spectroscopic techniques (UV–vis, fluorescence and CD) using human serum albumin (HSA) as a model protein. The complex displayed desired affinity to HSA in which hydrophobic interaction played a major role. In addition, the complex was subjected to cytotoxicity tests in vitro using three human cancer cells lines (HepG2, HeLa and A549) and showed prominent and selective cytotoxicity against HepG2 cell lines (IC50 ∼ 17.78 μM).

Graphical abstractThe DNA binding and cleavage properties of the copper(II) complex derived from 2-(2′-pyridyl)benzothiazole and glycylglycine were studied by spectroscopic methods, viscosity, and electrophoresis measurements. Further, the affinity of the complex to human serum albumin was investigated via multispectroscopic techniques, and the in vitro cytotoxicity was evaluated by MTT assay.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Copper(II)–dipeptide complex; 2-(2′-Pyridyl)benzothiazole; DNA binding; Nuclease activity; HSA interaction; Cytotoxicity
First Page Preview
Synthesis, characterization, DNA binding and cleavage, HSA interaction and cytotoxicity of a new copper(II) complex derived from 2-(2′-pyridyl)benzothiazole and glycylglycine
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 276, 15 February 2014, Pages 83–95
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering