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Erythromycin degradation by esterase (EreB) in enzymatic membrane reactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
2675 126 2016 9 PDF Available
Title
Erythromycin degradation by esterase (EreB) in enzymatic membrane reactors
Abstract

•Free and immobilized EreB exhibited an effective removal of Erythromycin.•Effect of pH and temperature on EreB activity.•Different enzymatic membrane reactor configurations for Erythromycin depletion.•Good stability of active membranes for erythromycin degradation in an enzymatic membrane reactor.

Erythromycin is an antibiotic considered as a recalcitrant pollutant because it is currently found in treated wastewaters. In addition, traces of antibiotics are a threatening ecological issue as these compounds are biologically active even at very low concentrations (ng L−1). In this work, we studied enzymatic degradation of this antibiotic in model aqueous solutions containing 20 mg L−1 erythromycin. We used as the biocatalyst an EreB esterase obtained from a genetically modified Escherichia coli W3110 strain carrying the EreB overexpression plasmid. Experiments were carried out with different reactor and biocatalyst configurations (free and immobilized by covalent grafting or adsorption). Free enzymes showed very high activity leading to erythromycin depletion. Experiments with the biocatalyst immobilized by adsorption using an enzymatic membrane reactor showed that even if EreB initially lost part of its activity, it was stabilized and able to continuously degrade erythromycin at a rate of 15.8 mg h−1 for 100 h.

Keywords
Enzymatic membrane reactor; Esterase immobilization; Erythromycin degradation; Ceramic membrane; Wastewater treatment
First Page Preview
Erythromycin degradation by esterase (EreB) in enzymatic membrane reactors
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 114, 15 October 2016, Pages 70–78
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering