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Green-emissive molecular marker with a TRIS-scaffold for fluorescence imaging of Zn2+ in biological systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
26762 43975 2014 7 PDF Available
Title
Green-emissive molecular marker with a TRIS-scaffold for fluorescence imaging of Zn2+ in biological systems
Abstract

•The probe L2 is Zn2+-specific and cell permeable.•Imaging of intracellular zinc (in vitro studies).•Real-time in vivo imaging of zinc in a whole plant Peperomia pellucida.•Detection and localization of zinc in Gram seed sprouts (in vivo studies).•L2 can detect zinc in nanomolar range (20 nM).

Three TRIS-based molecular probes, L1/L2/L3, were screened for their ability to detect Zn2+ at nanomolar level. Keeping in mind the criteria for a molecular probe to be utilized as bioimaging material, L2 was chosen as model compound for biological studies due to its higher excitation/emission wavelengths. While investigating the potential of L2, intracellular Zn2+ was successfully imaged using fluorescence microscopy. As the fluorescence emission of L2-Zn2+ ensemble was stable for longer duration, more than 3 h, L2 was successfully utilized in in vivo imaging of Zn2+ in a whole plant (Peperomia pellucida) in real-time. Further to its in vivo imaging capability, L2 was found to be successful in imaging zinc pool of gram seed sprouts.

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Keywords
Molecular probe; Fluorescence bioimaging; Gram seed sprouts; Intracellular detection; Zinc translocation
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Green-emissive molecular marker with a TRIS-scaffold for fluorescence imaging of Zn2+ in biological systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 277, 1 March 2014, Pages 75–81
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us