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Probing the DNA–ionic liquid interaction using an ultrafast molecular rotor

Paper ID Volume ID Publish Year Pages File Format Full-Text
26868 43983 2012 7 PDF Available
Title
Probing the DNA–ionic liquid interaction using an ultrafast molecular rotor
Abstract

The interaction of imidazolium based ionic liquids with calf thymus DNA has been investigated using a benzothiazole based ultrafast molecular rotor, Thioflavin-T, as a local reporter. It has been found that the addition of ionic liquid causes large reduction in the emission yield of the DNA bound probe indicating a strong interaction between the ionic liquids and DNA. Detailed studies show that the interaction between ionic liquids and DNA is sufficiently strong to remove the bound probe from the DNA. The excited state lifetime of the DNA bound probe measured by femtosecond fluorescence upconversion technique also corroborate with the above proposition. To understand the nature of the interaction between ionic liquids and DNA, photophysical properties of Thioflavin-T in DNA was investigated in the presence of imidazolium based ionic liquids having alkyl side chain of different lengths in the cationic moiety and the results indicate that apart from electrostatic interaction, the hydrophobicity of ionic liquid also plays a significant role in the ionic liquid–DNA interaction.

Graphical abstractDNA–ionic liquid interaction.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Use of ultrafast molecular rotor to study the interaction between ionic liquids and DNA. ► Ultrafast molecular rotor is highly sensitive to detect a small changes in the DNA structure due to its interaction with ionic liquids. ► Along with electrostatic interaction, hydrophobic interaction plays important role in ionic liquid–DNA interaction.

Keywords
Ultrafast molecular rotor; Ionic liquid; DNA; Fluorescence; Time-resolved spectroscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 246, 15 October 2012, Pages 16–22
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us