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Application of Euler–Lagrange CFD for quantitative evaluating the effect of shear force on Carthamus tinctorius L. cell in a stirred tank bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
2691 126 2016 9 PDF Available
Title
Application of Euler–Lagrange CFD for quantitative evaluating the effect of shear force on Carthamus tinctorius L. cell in a stirred tank bioreactor
Abstract

•Hydrodynamics environment was quantified by CFD analysis and validated by PIV.•An online capacitance electrode was used to count the dynamic shear effects.•A new Euler–Lagrange based parameter was successfully developed.

The relationship between shear environment and physiological characteristics of plant cells is always a crucial and challenging aspect in the scale up of suspension cultivations. In this work, we propose a quantitative method for evaluating the lethal effects of hydrodynamic on Carthamus tinctorius L. cell with computational fluid dynamic (CFD) technology and online capacitance viable cell detection. The study towards the effect of short term shear force on C. tinctorius L. cells was carried out in 5 L bioreactor under various overall shear intensities (0.02–1.82 w/kg), in which an online capacitance electrode was applied to count the dynamic vital cell. A first order kinetic model was achieved to describe the death rate in a time span of 60 min based on the capacitance. A product of the maximum shear stress and shear frequency (SSF) parameter, which is an evolution of energy dissipation/circulation function, was established to relate cell death rate to the shear environment. A good correlation between cell death kinetics and the SSF parameter was observed in the verification test in a 15 L STR bioreactor. SSF can be used to determine the maximum operating range of hydrodynamic stress, and improve the design and optimization of the of C. tinctorius L. cultivation in scale-up process.

Keywords
D, impeller diameter (mm); T, tank diameter (mm); EDR, energy dissipation rate (m2 s−3 or w kg−1); H, liquid high (mm); N, agitation speed (rpm revolutions per minute); OUR, oxygen uptake rate (mmol kg−1 h−1); CER, carbon dioxide evolution rate (mmol kg−1
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Application of Euler–Lagrange CFD for quantitative evaluating the effect of shear force on Carthamus tinctorius L. cell in a stirred tank bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 114, 15 October 2016, Pages 209–217
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us