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Fluorescence behavior of ethidium bromide in homogeneous solvents and in presence of bile acid hosts

Paper ID Volume ID Publish Year Pages File Format Full-Text
26922 43987 2012 9 PDF Available
Title
Fluorescence behavior of ethidium bromide in homogeneous solvents and in presence of bile acid hosts
Abstract

Photoluminescence behavior of ethidium bromide (EB) was studied in homogeneous solvents as well as in presence of bile acid surfactants. A quantitative analysis based on Kamlet–Taft equation toward the contribution of different solvatochromic parameters like solvent polarizability (π*), hydrogen bond donor (α) and hydrogen bond acceptor (β) ability, on EB spectral properties reveals that though fluorescence quantum yield and lifetime do not show any straight forward correlation individually, the substantial decrease in these quantities is due to the increase in total nonradiative decay rate in polar protic medium. The interaction of EB with bile acid hosts gives rise to large increase in fluorescence intensity due to shielding of the probe in less hydrogen bonding environment. Global analysis of the fluorescence decay behavior indicates that more than 50% of the fluorophores get sequestered inside the micellar sub-domain with an apparent binding constant of ∼103 M−1.

Graphical abstractThe increase in ethidium bromide fluorescence intensity and lifetime in presence of bile acid hosts is due to the shielding of the sequestered probes from polar and hydrogen bonding aqueous environment.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Quantitative contribution of solvent parameters on ethidium bromide (EB) fluorescence solvatochromism. ► Correlation of Kamlet–Taft parameters with total non-radiative decay rate. ► Steady state and time-resolved analysis of fluorescence quenching in different mixed solvent systems. ► Interaction of EB with three different bile acids, viz. cholic acid (CA), deoxycholic acid (DCA) and chenodeoxycholic acid (CDCA). ► Strong interaction of EB with bile acids in comparison to the other surfactants due to facial amphiphilicity.

Keywords
Fluorescence solvatochromism; Ethidium bromide; Fluorescence quenching; Bile acid; Global analysis
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Fluorescence behavior of ethidium bromide in homogeneous solvents and in presence of bile acid hosts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 244, 15 September 2012, Pages 9–17
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us