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Modelling of batch kinetics of aerobic carotenoid production using Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
2693 126 2016 11 PDF Available
Title
Modelling of batch kinetics of aerobic carotenoid production using Saccharomyces cerevisiae
Abstract

•Growth kinetics of genetically modified Saccharomyces cerevisiae were examined.•Models were proposed to predict production of carotenoids and other metabolites.•Local and global sensitivity were analyzed to determine quality of models.•Models were able to accurately predict overflow metabolism of modified yeast strain.

Carotenes such as β-carotene have a positive impact in human health as a precursor of vitamin A. The structural complexity of these compounds makes chemical synthesis a difficult option, facilitating the need for their biological production. Reliable mathematical models for batch cultivation are developed to describe the glucose consumption, product formation and depletion, and β-carotene production of the Saccharomyces cerevisiae strain mutant SM14 with 20 g/L glucose as the carbon source. Parameter estimation for the models employ an objective function minimizing an expression that uses the coefficient of determination R2R2, which avoids the necessity of weighting or normalizing the equations. Comparison with experimental data shows that the developed models show a satisfactory prediction of the overflow metabolism that is happening in the cell. Additionally, local and global sensitivity analysis of the models with respect to the optimal parameters is also studied and further show that the models developed here accurately describe trends in the dynamic states of the bioreactor during batch fermentation.

Keywords
Batch processing; β-Carotene; Modelling; Yeast cultivation; Sensitivity analysis
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Modelling of batch kinetics of aerobic carotenoid production using Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 114, 15 October 2016, Pages 226–236
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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