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Photoactive spatial proximity probes for binding pairs with epigenetic marks

Paper ID Volume ID Publish Year Pages File Format Full-Text
26945 43988 2014 8 PDF Available
Title
Photoactive spatial proximity probes for binding pairs with epigenetic marks
Abstract

•Photoassisted binding assay differentiates peptides with epigenetic marks.•The library-encoding strategy is compatible with automated peptide synthesis.•Methodology could be adapted for assaying solution phase libraries.

A new strategy for encoding polypeptide libraries with photolabile tags is developed. The photoassisted assay, based on conditional release of encoding tags only from bound pairs, can differentiate between peptides which have minor differences in a form of post-translational modifications with epigenetic marks. The encoding strategy is fully compatible with automated peptide synthesis. The encoding pendants are compact and do not perturb potential binding interactions.

Keywords
Encoded combinatorial library; Spatial proximity test; Conditional photorelease of tags; Epigenetic marks
First Page Preview
Photoactive spatial proximity probes for binding pairs with epigenetic marks
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 290, 15 September 2014, Pages 101–108
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering