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Sensitive ECL sensor for sequence-specific DNA from Mycobacterium tuberculosis based on N-(aminobutyl)-N-ethylisoluminol functionalized gold nanoparticles labeling

Paper ID Volume ID Publish Year Pages File Format Full-Text
26957 43989 2012 7 PDF Available
Title
Sensitive ECL sensor for sequence-specific DNA from Mycobacterium tuberculosis based on N-(aminobutyl)-N-ethylisoluminol functionalized gold nanoparticles labeling
Abstract

A novel sensor was developed for highly sensitive detection of the sequence-specific DNA of Mycobacterium tuberculosis (MTB) by using N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functionalized gold nanoparticles (ABEI-AuNPs) as labels and using 81 bp segment derived from the MTB specific insertion sequence IS6110 as the target strand. Owing to the novel ECL labels and amplification of gold nanoparticles and biotin–streptavidin system, extremely high sensitivity for ss-DNA could be achieved with a low detection limit of 3.3 × 10−16 M and a wide linear dynamic range of 1.0 × 10−15–1.0 × 10−12 M. The DNA sensor also showed good precision, good stability, low detection limit for the detection of sequence-specific DNA from MTB in serum samples, which might be of application potential in clinical diagnosis.

► A DNA ECL sensor without PCR amplification is developed for the detection of MTB. ► This method uses N-(aminobutyl)-N-(ethylisoluminol) functionalized gold nanoparticles as labels. ► The sensor can achieve extremely high sensitivity. ► It is of great application potential in clinical diagnosis.

Keywords
Tuberculosis; Electrochemiluminescence; DNA sensor; N-(aminobutyl)-N-ethylisoluminol functionalized gold nanoparticles
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Sensitive ECL sensor for sequence-specific DNA from Mycobacterium tuberculosis based on N-(aminobutyl)-N-ethylisoluminol functionalized gold nanoparticles labeling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 241, 1 August 2012, Pages 45–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us