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pH dependence of the fluorescence lifetime of enhanced yellow fluorescent protein in solution and cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
26999 43992 2012 7 PDF Available
Title
pH dependence of the fluorescence lifetime of enhanced yellow fluorescent protein in solution and cells
Abstract

pH dependence of the fluorescence decay profile of enhanced yellow fluorescent protein (EYFP) depends on the excitation wavelength. The correlation between the fluorescence lifetime and pH in solution is discussed in terms of the acid–base equilibrium of the chromophore of EYFP. Fluorescence lifetime images of EYFP in HeLa cells have also been measured at various values of intracellular pH. A remarkable pH dependence of the fluorescence lifetime image is observed in pH 4.5–6.0, indicating that pH in a single cell can be evaluated using the fluorescence lifetime image of EYFP especially in the acidic condition.

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Keywords
EYFP; Intracellular pH; Fluorescence decay profile; Fluorescence lifetime image; HeLa cells
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pH dependence of the fluorescence lifetime of enhanced yellow fluorescent protein in solution and cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 235, 1 May 2012, Pages 65–71
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us