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Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning using decellularized matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
270 22 2015 12 PDF Available
Title
Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning using decellularized matrix
Abstract

As a tissue-specific stem cell for chondrogenesis, synovium-derived stem cells (SDSCs) are a promising cell source for cartilage repair. However, a small biopsy can only provide a limited number of cells. Cell senescence from both in vitro expansion and donor age presents a big challenge for stem cell based cartilage regeneration. Here we found that expansion on decellularized extracellular matrix (dECM) full of three-dimensional nanostructured fibers provided SDSCs with unique surface profiles, low elasticity but large volume as well as a fibroblast-like shape. dECM expanded SDSCs yielded larger pellets with intensive staining of type II collagen and sulfated glycosaminoglycans compared to those grown on plastic flasks while SDSCs grown in ECM yielded 28-day pellets with minimal matrix as evidenced by pellet size and chondrogenic marker staining, which was confirmed by both biochemical data and real-time PCR data. Our results also found lower levels of inflammatory genes in dECM expanded SDSCs that might be responsible for enhanced chondrogenic differentiation. Despite an increase in type X collagen in chondrogenically induced cells, dECM expanded cells had significantly lower potential for endochondral bone formation. Wnt and MAPK signals were actively involved in both expansion and chondrogenic induction of dECM expanded cells. Since young and healthy people can be potential donors for this matrix expansion system and decellularization can minimize immune concerns, human SDSCs expanded on this future commercially available dECM could be a potential cell source for autologous cartilage repair.

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Keywords
Decellularized extracellular matrix; Synovium-derived stem cell; Chondrogenesis; Endochondral bone formation; Atomic force microscopy
First Page Preview
Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning using decellularized matrix
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 20, 1 July 2015, Pages 39–50
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering