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Synthesis and photodynamic properties of 5,10,15,20-tetrakis[3-(N-ethyl-N-methylcarbazoyl)]chlorin and its analogous porphyrin in solution and in human red blood cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
27053 43997 2014 9 PDF Available
Title
Synthesis and photodynamic properties of 5,10,15,20-tetrakis[3-(N-ethyl-N-methylcarbazoyl)]chlorin and its analogous porphyrin in solution and in human red blood cells
Abstract

•A novel tetracationic meso-substituted carbazoyl chlorin was synthesized.•Tetracationic chlorin produces efficiently singlet molecular oxygen.•In presence of NADH, chlorin photosensitizes the formation of superoxide anion radical.•A high photohemolytic activity was found for chlorin in the presence of erythrocytes.

The spectroscopic and the photodynamic properties of 5,10,15,20-tetrakis[3-(N-ethyl-N-methylcarbazoyl)]chlorin (TEMCC) and its analogous porphyrin (TEMCP) were investigated in N,N-dimethylformamide (DMF) and in human red blood (HRB) cells. A more intense absorption band at 650 nm was obtained for TEMCC in comparison with TEMCP. Both photosensitizers showed red fluorescence emission with quantum yields of 0.13 and 0.23 for TEMCP and TEMCC, respectively. The photodynamic activity of TEMCC was higher that TEMCP in DMF, with a quantum yield of singlet molecular oxygen of 0.49. Moreover, in presence of NADH these photosensitizers produced superoxide anion radical. In vitro experiments showed that TEMCC was a highly effective agent to hemolyze the HRB cells. A decrease in the HRB cells osmotic stability was observed after photodynamic treatment with TEMCC. Studies of photodynamic action mechanism showed that the photohemolysis of HRB cells was protected in the presence of azide ion. Also, a reduction on the cell photodamage was found using mannitol. In contrast, the photohemolytic effect considerably increased in D2O. Therefore, the photodynamic activity of TEMCC in HRB cells was mediated by a contribution of type I and type II photooxidative mechanisms.

Graphical abstractSpectroscopic and photodynamic properties of a novel chlorin derivative bearing four carbazoyl units were studied in solution and in human erythrocytes.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Chlorin; Carbazole; Photodynamic activity; Erythrocytes; Photohemolysis
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Synthesis and photodynamic properties of 5,10,15,20-tetrakis[3-(N-ethyl-N-methylcarbazoyl)]chlorin and its analogous porphyrin in solution and in human red blood cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 282, 15 May 2014, Pages 16–24
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us