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FISHji: New ImageJ macros for the quantification of fluorescence in epifluorescence images

Paper ID Volume ID Publish Year Pages File Format Full-Text
2707 127 2016 9 PDF Available
Title
FISHji: New ImageJ macros for the quantification of fluorescence in epifluorescence images
Abstract

•Five image analysis methods to quantify fluorescence intensity were proposed.•FISHji3, FISHji4 and FISHji5 showed high accuracy.•Automatic FISHji methods allowed a fast and easy quantification of fluorescence.

Fluorescence in situ hybridization (FISH) is based on the use of fluorescent staining dyes, however, the signal intensity of the images obtained by microscopy is seldom quantified with accuracy by the researcher. The development of innovative digital image processing programs and tools has been trying to overcome this problem, however, the determination of fluorescent intensity in microscopy images still has issues due to the lack of precision in the results and the complexity of existing software.This work presents FISHji, a set of new ImageJ methods for automated quantification of fluorescence in images obtained by epifluorescence microscopy. To validate the methods, results obtained by FISHji were compared with results obtained by flow cytometry. The mean correlation between FISHji and flow cytometry was high and significant, showing that the imaging methods are able to accurately assess the signal intensity of fluorescence images. FISHji are available for non-commercial use at http://paginas.fe.up.pt/∼nazevedo/.

Keywords
FISH, fluorescence in situ hybridization; LNA, locked nucleic acid; LoG, Laplacian of Gaussian; MFI, mean fluorescence intensity; PI, propidium iodide; ROIs, regions of interest; TSA, trypticase soy agar; 2′OMe, 2′-O-methly RNABiomedical; RNA; LNA; Image
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FISHji: New ImageJ macros for the quantification of fluorescence in epifluorescence images
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 112, 15 August 2016, Pages 61–69
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us