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Using an innovative pH-stat CO2 feeding strategy to enhance cell growth and C-phycocyanin production from Spirulina platensis

Paper ID Volume ID Publish Year Pages File Format Full-Text
2709 127 2016 8 PDF Available
Title
Using an innovative pH-stat CO2 feeding strategy to enhance cell growth and C-phycocyanin production from Spirulina platensis
Abstract

•C-Phycocyanin is produced by Spirulina platensis with simultaneous CO2 biofixation.•A pH-stat CO2 feeding strategy improves efficiency of CO2 biofixation.•Two-step C-PC purification achieved 33% recovery and 4.33 purity (A615/A280).•C-PC purity of 4.33 is analytical grade and has commercial potential.

Spirulina platensis can mitigate CO2 emissions and simultaneously produce an antioxidant called C-phycocyanin (C-PC), which is a high value nutraceutical product. An innovative pH control system was used to cultivate S. platensis, in which CO2 feeding, instead of acid/alkaline titration, was used to control the pH of the culture. The optimum culture pH was 9.5. Compared to continuous CO2 feeding system, the pH control system improved the CO2 removal efficiency from 13.6% to 62.3%. The C-PC content and productivity were also enhanced to 16.8% and 0.17 g/L/d, respectively. Therefore, the proposed pH control system is economic and sustainable as it avoids the use of acid/alkaline and reduces the overall CO2 emissions. A two-stage C-PC purification process combining fractional precipitation and ion exchange chromatography could achieve the highest purity (A615/A280) of 4.33 with a 33% C-PC recovery.

Keywords
Microalgae; C-Phycocyanin; pH control system; Chromatography; Optimization; Purification
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Using an innovative pH-stat CO2 feeding strategy to enhance cell growth and C-phycocyanin production from Spirulina platensis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 112, 15 August 2016, Pages 78–85
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us