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Europium-sensitized and simultaneous pH-assisted spectrofluorimetric assessment of ciprofloxacin, norfloxacin and gatifloxacin in pharmaceutical and serum samples

Paper ID Volume ID Publish Year Pages File Format Full-Text
27182 44007 2012 9 PDF Available
Title
Europium-sensitized and simultaneous pH-assisted spectrofluorimetric assessment of ciprofloxacin, norfloxacin and gatifloxacin in pharmaceutical and serum samples
Abstract

A facile, ultrasensitive and interference free europium-sensitized spectrofluorimetric method was developed for the simultaneous determination of ciprofloxacin (CFLX), norfloxacin (NFLX), and gatifloxacin (GFLX) in their mixture. A coordination complexes of Eu3+–CFLX/NFLX/GFLX were formed and the coordination was determined using the mole ratio method of continuous variation of equimolar solutions. It was found that the luminescence intensity of Eu3+ for these complexes in their mixtures is quite sensitive to three variant sets of pH, 6.0, 5.5 and 3.5 and excitation wavelengths (λex), 365, 340 and 395 for CFLX, NFLX and GFLX, respectively. Thus a dual-controlled luminescence of smoothly dynamic reversibility is achieved where a reversible on/off switchable emission of one system was observed by tuning its optimal values of pH and λex to the optimal ones of the second and so on for the third. The monitored luminescence intensity of the system showed a good linear relationship with the concentration of CFLX over the range of 5 × 10−8 to 1 × 10−6 mol L−1 with a correlation coefficient of 0.995, for NFLX within a range of 5 × 10−8 to 1.8 × 10−6 mol L−1 with a correlation coefficient of 0.990, and for GFLX within a range of 5 × 10−8 to 1.2 × 10−6 mol L−1 with a correlation coefficient of 0.995. The detection limit (LOD) was determined as 1.5 × 10−8 mol L−1 for CFLX, 3.0 × 10−8 mol L−1 for NFLX and 1.6 × 10−8 mol L−1 for GFLX. The limit of quantification (LOQ) is 4.5 × 10−8, 9.0 × 10−8, 2.8 × 10−8 for CFLX, NFLX and GFLX, respectively. Moreover, a ternary mixture of CFLX, NFLX and GFLX was satisfactorily assessed with average error <4.5%. This method has been successfully validated to the simultaneous and selective determination of CFLX, NFLX and GFLX in pharmaceuticals and serum samples with recoveries of (100.2 ± 2.1, 99.6 ± 2.5 and 103.1 ± 2.9) and (102.9 ± 1.1, 102.5 ± 1.5 and 104.4 ± 3.9), respectively.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A simultaneous determination of (CFLX), (NFLX), and (GFLX) based on europium-sensitized and pH-assisted. ► The highest luminescence intensity of the Eu3+–CFLX/NFLX or GFLX solution was observed in acetonitrile. ► The luminescence of Eu3+ complexes with mixture of CFLX, NFLX and GFLX is sensitive to three variant sets of pH and λex. ► For Eu3+–(CFLX), (NFLX), and (GFLX), λex of 365, 340 and 395 nm and pH 6.0, 5.5 and 3.5, respectively.

Keywords
Ciprofloxacin; Norfloxacin; Gatifloxacin; Luminescence; Energy transfer; Europium sensitized
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Europium-sensitized and simultaneous pH-assisted spectrofluorimetric assessment of ciprofloxacin, norfloxacin and gatifloxacin in pharmaceutical and serum samples
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 236, 15 May 2012, Pages 26–34
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us