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Two consecutive step process for ethanol and microbial oil production from sweet sorghum juice

Paper ID Volume ID Publish Year Pages File Format Full-Text
2722 127 2016 7 PDF Available
Title
Two consecutive step process for ethanol and microbial oil production from sweet sorghum juice
Abstract

•Ethanol and microbial oil consecutive production from sweet sorghum juice.•Ethanol is produced first.•Oleaginous yeast growth employing the residual carbon and nitrogen left.•Glucose had the highest uptake rate.•Fructose was slowly metabolized and a substantial amount remained.

The juice extracted from sweet sorghum stalks has been previously explored to produce ethanol and also to grow oleaginous yeasts and algae with the objective of producing microbial oil. In this paper we propose a different process route in order to produce ethanol and microbial oil in two consecutive fermentation steps. Ethanol is produced first followed by the growth of oleaginous yeast employing the residual carbon and nitrogen left from the first step. Two yeasts were compared for ethanol production. Trichosporon oleaginosus was cultivated for lipid production. The yeast selection for the first step was the most important factor for achieving a high ethanol yield and the effect of inorganic nitrogen addition was not significant. The remaining sugars consisted of a mixture of sucrose and fructose and no residual glucose was detected in any of the runs. In the second step T. oleaginosus DSM 11815 grew in the pooled juices remaining from the first step for 168 h and produced biomass with 28% lipid content. Glucose showed the highest uptake rate, sucrose was utilized until low glucose values prevailed, and fructose was slowly metabolized and a substantial amount remained. Although the two step process has flexibility in choosing the proper microorganism for each step, it is necessary to look for a rapid fructose uptake strain for both fermentations.

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Keywords
Bioprocess design; Ethanol; Fermentation; Consolidated processing; Microbial oil; Sweet sorghum
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Two consecutive step process for ethanol and microbial oil production from sweet sorghum juice
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 112, 15 August 2016, Pages 186–192
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us