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Adsorption and photocatalytic degradation of human serum albumin on TiO2 and Ag–TiO2 films

Paper ID Volume ID Publish Year Pages File Format Full-Text
27372 44018 2011 9 PDF Available
Title
Adsorption and photocatalytic degradation of human serum albumin on TiO2 and Ag–TiO2 films
Abstract

Titanium dioxide is a useful material in the biomedical field as it has excellent biocompatibility based on its non-toxicity and non-inflammatory properties. Furthermore, TiO2 can be excited by UV light to create charge carriers giving rise to photocatalytic redox reactions at the surface and photo-induced super-hydrophilicity. These properties might be exploited for surface decontamination of medical devices and implants. With this in mind, titanium dioxide TiO2 films were prepared on stainless steel substrates using magnetron sputtering. Silver loaded (Ag–TiO2) films were prepared by the photocatalytic reduction of Ag+ from solution. The adsorption of human serum albumin (HSA) was studied. Surface analysis methods used included X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and atomic force microscopy (AFM). The TiO2 films were predominantly anatase crystal phase and the photoreduced Ag was present at greater than 90% of the silver content as Ag0 on the surface. Ag loading of the TiO2 markedly enhanced the Raman signal (ca. 15-fold), but caused significant changes to the spectrum indicating non-specific binding of protein side chain residues to the Ag. The amide I and III modes remained well-resolved and were used to estimate the conformational change induced by the Ag. Raman analysis showed an increase in the intensity of the band at ∼1665 cm−1 assigned to the disordered conformation, suggesting that the adsorption to the Ag sites induces conformational changes in the protein. UVB irradiation of the protein contaminated surfaces caused further changes in the protein conformation, consistent with denaturation and enhanced binding and oxidation, thought to be induced through a photocatalytic mechanism.

► TiO2 synthesised and modified with Ag nano clusters. ► Adsorption study using Human serum albumin and Investigation of protein configuration via Raman, FTIR, XPS spectroscopes. ► HSA was subsequently degraded by photocatalysis. ► Mechanism of degradation investigated change due to esterification of carboxyl species.

Keywords
TiO2; Ag–TiO2; Photocatalytic activity; Protein; Adsorption; Degradation
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Adsorption and photocatalytic degradation of human serum albumin on TiO2 and Ag–TiO2 films
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 222, Issue 1, 5 July 2011, Pages 123–131
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us