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Quantitative evaluation of the shear threshold on Carthamus tinctorius L. cell growth with computational fluid dynamics in shaken flask bioreactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
2741 129 2016 11 PDF Available
Title
Quantitative evaluation of the shear threshold on Carthamus tinctorius L. cell growth with computational fluid dynamics in shaken flask bioreactors
Abstract

•Hydrodynamic stress in shaken flask was quantitively analyzed through CFD simulation.•Effects of shear stress on the growth and morphological were investigated.•A threshold was observed at average and maximum shear stress.

Quantitative evaluation of the shear threshold on C. tinctorius L. cell growth is vital for bioreactor system design and optimization of scaled-up industrial cultivation. The present research focused on investigating the effects of shear force on C. tinctorius L. cell growth by computational fluid dynamic (CFD) analysis in shaken flasks. The results revealed that specific cell growth rates were greatly inhibited as the shear force increased from 1.17 to 2.42 Pa. Recovery of viability and aggregation diameter to their normal levels could be implemented after a 4-day adaptation with large fluctuations in physiological state. With further correlation analysis on shear force and C. tinctorius L. growth rate, a threshold value was identified at an average and maximum shear stress of approximately 0.55 Pa (0.06 w/kg) and 4.00 Pa (0.93 w/kg) according to the influence on cell growth. Quantitative data on shear effects can facilitate the design of industrial processes and lead to more rational scale-up in industrial C. tinctorius L. cultivation.

Keywords
a, specific gas-liquid interfacial area (m2); c*, saturated dissolved oxygen (mmol/m3); co, concentration of oxygen in liquid (mmol/m3); d, largest inner diameter of shake flask (mm); d0, shaking diameter (mm); kLa, mass transfer coefficient (h−1); n, sha
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Quantitative evaluation of the shear threshold on Carthamus tinctorius L. cell growth with computational fluid dynamics in shaken flask bioreactors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 113, 15 September 2016, Pages 66–76
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us