fulltext.study @t Gmail

Non-toxic fluorescent alanine–fluorescein probe with green emission for dual colorimetric/fluorimetric sensing

Paper ID Volume ID Publish Year Pages File Format Full-Text
27425 44022 2013 10 PDF Available
Title
Non-toxic fluorescent alanine–fluorescein probe with green emission for dual colorimetric/fluorimetric sensing
Abstract

•Nontoxic fluorescent probes derived from alanine–fluorescein.•Cytoxicity, fluorescence spectroscopy, and colorimetric assays were used.•Mercury(II) fluorescent probes.

Two new fluorescent bio-inspired dyes 1 and 2 containing a fluorescein unit linked to a protected with a Boc group (N-tert-butyloxycarbonyl), and unprotected alanine residue were synthesized and characterized. The acid–base behavior was studied in water/ethanol (60/40) and at pH = 8 a strong emission fluorescence, followed by a color change from colorless to yellow was observed. Photophysical studies in water HEPES buffered solution toward alkaline, alkaline earth, transition, and post- transition metal ions were performed. Compounds 1 and 2 revealed to be very sensitive to Hg2+ with colorimetric changes from yellow to pink in the presence of HEPES buffer. Moreover, compound 1 show a color change from yellow to orange in the presence of the neurotransmitter molecule dopamine. Compound 2 also reveals to be sensitive to Cu2+ and Ni2+, being able to detected and quantified in HEPES buffer ca. 0.37 ppm and ca. 1.50 ppm of Cu2+, respectively. Very small silver nanoparticles coated with compound 2 of ca. 3.68 ± 0.93 nm were successfully synthesized and characterized. Toxicological studies with both compounds were also carried out.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Fluorescein; Dopamine; Fluorescent chemosensors; Alanine; Hg2+; Toxicity studies
First Page Preview
Non-toxic fluorescent alanine–fluorescein probe with green emission for dual colorimetric/fluorimetric sensing
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 269, 1 October 2013, Pages 17–26
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering