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Biodiesel production using magnetic whole-cell biocatalysts by immobilization of Pseudomonas mendocina on Fe3O4-chitosan microspheres

Paper ID Volume ID Publish Year Pages File Format Full-Text
2743 129 2016 7 PDF Available
Title
Biodiesel production using magnetic whole-cell biocatalysts by immobilization of Pseudomonas mendocina on Fe3O4-chitosan microspheres
Abstract

•First report on Pseudomonas mendocina containing MWCBs for lipid conversion.•A biodiesel yield of 87.32% was achieved at optimized conditions within 48 h.•MWCBs prepared can be reused for 10 cycles with retaining 83.57% yield.•Kinetic data followed Michaelis-Menten model with methanol competitive inhibition.

Magnetic whole-cell biocatalysts (MWCBs) for enzymatic biodiesel production from soybean oil were constructed by immobilizing Pseudomonas mendocina cells into Fe3O4-chitosan microspheres. The effects of the MWCBs concentration, temperature, the molar ratio of methanol to oil and water content on biodiesel yield were studied. For the MWCBs, a biodiesel yield of 87.32% was obtained under the optimum operating condition (MWCBs concentration of 10 wt.%, water content of 10 wt.%, 35 °C, methanol to oil molar ratio of 4:1 and a four-step addition of methanol) for 48 h. The kinetic data followed Michaelis-Menten model (rmax, 2.1801 mg/min; Km, 0.4272 mg/mL; KI, 0.2308 mg/mL) with competitive inhibition by methanol. MWCBs had an excellent reusability and still gave a biodiesel yield of 83.57% after 10 cycles, which was higher than that of Fe3O4-uncontained whole cell biocatalysts (74.06%). Moreover, MWCBs could be separated and be recycled easily due to their superparamagnetism. Therefore, MWCBs would be promising for large-scale biodiesel industry.

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Keywords
Biodiesel; Pseudomonas mendocina; Magnetic whole-cell biocatalyst; Transesterification; Immobilization
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Biodiesel production using magnetic whole-cell biocatalysts by immobilization of Pseudomonas mendocina on Fe3O4-chitosan microspheres
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 113, 15 September 2016, Pages 86–92
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us