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Spectroscopic parameters of phycoerythrobilin and phycourobilin on phycoerythrin from Gracilaria chilensis

Paper ID Volume ID Publish Year Pages File Format Full-Text
27461 44024 2011 6 PDF Available
Title
Spectroscopic parameters of phycoerythrobilin and phycourobilin on phycoerythrin from Gracilaria chilensis
Abstract

The high efficiency of energy transfer by phycobiliproteins in phycobilisomes is an interesting and exciting biological phenomenon. The phycobilisome of Gracilaria chilensis contains three phycobiliproteins: phycoerythrin, phycocyanin and allophycocyanin. These proteins have their absorption and emission spectra overlapped allowing a non-radiative, direct and efficient transfer of the excitation energy among them, which is channelled along an energy gradient from the rods to the core and finally transferred to chlorophyll a. Phycobiliproteins are highly fluorescent proteins due to the presence of covalently bound phycobilins which provides them their functional properties. This work reports the experimental determination of the spectroscopic parameters for the chromophores present in R-phycoerythrin of G. chilensis: lifetime, quantum yield, and extinction coefficient of phycourobilin and phycoerythrobilin, using chromophorylated tryptic fragments containing one or two chromophores. These fragments were identified by mass spectrometry and characterized spectroscopically. These measurements provide the individual spectroscopic parameters of the three chromophores (two phycoerythrobilins and one phycourobilin) in the β subunit and the two chromophores (phycoerythrobilins) bound to the α subunit considering the protein environment.

Research highlights► The spectroscopic parameters of phycourobilin and phycoerythrobilin on phycoerythrin were determined. Tryptic chromophorylated fragments of the protein were used to simulate the protein environment and avoid interference with other chromophores in the molecule.

Keywords
PBS, phycobilisome; PE, phycoerythrin; PC, phycocyanin; APC, allophycocyanin; FRET, Fluorescence Energy Transfer; λmaxA, maximum absorption wavelength; ɛ, extinction coefficient; λmaxE, maximum fluorescence emission wavelength; ϕ, fluorescence quantum yie
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Spectroscopic parameters of phycoerythrobilin and phycourobilin on phycoerythrin from Gracilaria chilensis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 219, Issues 2–3, 15 April 2011, Pages 211–216
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us