fulltext.study @t Gmail

Oxidative DNA damage following photoexcitation of daunomycin: Direct role of oxygen

Paper ID Volume ID Publish Year Pages File Format Full-Text
27484 44026 2008 5 PDF Available
Title
Oxidative DNA damage following photoexcitation of daunomycin: Direct role of oxygen
Abstract

The chemistry of the photoactivation of daunomycin–DNA complexes is reported and the mechanism is elucidated. We quantitatively assessed the type of DNA damage, such as strand breaks, oxidized bases, and abasic sites, that arise using a plasmid relaxation assay coupled with DNA repair endonucleases. Photoexcitation of daunomycin leads to oxidative DNA damage in a dose- and irradiation time-dependent manner and guanine-specific oxidized purines are substantially produced under these conditions. Oxidative DNA base damage was also inhibited by argon degassing, indicating that guanine-specific damage arises from an oxygen-dependent mechanism. In addition, photoexcitation of daunomycin–DNA complexes leads to superoxide anion radical formation. From these studies of the actual product formed, we conclude that a charge transfer is a main driving force of the mechanism.

Keywords
DM, Daunomycin; DNA, deoxyribonucleic acid; hOGG1, human 8-oxoguanine DNA glycosylase 1; Endo III, endonuclease III; APE1, human apurinic endonuclease 1; 8-oxodG, 8-oxo-7,8-dihydro-2′-deoxyguanosine; ROS, reactive oxygen species; CT, calf thymus; SOD, sup
First Page Preview
Oxidative DNA damage following photoexcitation of daunomycin: Direct role of oxygen
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 198, Issues 2–3, 15 August 2008, Pages 200–204
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us