fulltext.study @t Gmail

Effect of salts on the excited state of pyranine as determined by steady-state fluorescence

Paper ID Volume ID Publish Year Pages File Format Full-Text
27493 44026 2008 6 PDF Available
Title
Effect of salts on the excited state of pyranine as determined by steady-state fluorescence
Abstract

Pyranine is a pH-sensitive fluorescent probe useful in the pH range of 4.5–8, and it has been extensively employed to determine pH inside cells, membranes and membrane models. The fluorescent properties of pyranine are a consequence of the excited states ROH* and RO−*. The prototropic equilibrium of these excited species has a pKa* much lower than that of the ground state. In this paper we determined the pKa* (1.42 ± 0.06) and the relative quantum yield of pyranine in the pH range of 1–8 by analyzing the component peaks of the steady-state of the dye's emission spectrum. As pyranine is very sensitive to the medium we studied the influence of salts formed by mono-, di-, and trivalent ions on the apparent pKa*. In all cases, the presence of salts reduced the apparent pKa* to varying degrees depending on the valence of the cations. The strategy used to obtain this information was a dual emission ratiometric method at 441 and 511 nm after excitation at 350 nm. The results obtained demonstrate that pyranine is suitable to determine the pH of aqueous solutions in the range of 1–3.5.

Keywords
HPTS; pH probe; pKa determination; Salts; Spectral analysis
First Page Preview
Effect of salts on the excited state of pyranine as determined by steady-state fluorescence
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 198, Issues 2–3, 15 August 2008, Pages 262–267
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us