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Fabrication of enzyme-entrapped composite and macroporous gel beads by suspension gelation combined with sedimentation polymerization

Paper ID Volume ID Publish Year Pages File Format Full-Text
2751 129 2016 6 PDF Available
Title
Fabrication of enzyme-entrapped composite and macroporous gel beads by suspension gelation combined with sedimentation polymerization
Abstract

•Lipase-entrapped composite and macroporous gel beads were prepared.•Suspension gelation and sedimentation polymerization were combined.•The gels successfully catalyzed the hydrolysis of p-nitrophenyl acetate.

Composite and macroporous gel beads for the immobilization of enzymes were produced by combining suspension gelation with sedimentation polymerization. Physically cross-linked calcium alginate microcapsules containing lipase were prepared by electrostatic atomization. Monodisperse millimeter-sized poly(ethylene glycol) diacrylate hydrogel (composite gel) beads were fabricated by polymerizing droplets of pre-gel aqueous suspension that contained microcapsules during their descent in silicone oil. Macroporous gel beads with entrapped lipase were prepared by treating the composite gel beads with a trisodium citrate aqueous solution. The lipase immobilized within the composite and macroporous gels successfully catalyzed the hydrolysis of p-nitrophenyl acetate over successive cycles of use. The kinetics of the enzymatic reaction was analyzed using the Michaelis–Menten equation.

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Keywords
Immobilized lipase; In situ entrapment; Macroporous gel beads; Microcapsule; Suspension; Sedimentation polymerization
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Fabrication of enzyme-entrapped composite and macroporous gel beads by suspension gelation combined with sedimentation polymerization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 113, 15 September 2016, Pages 152–157
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us