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Megakaryocyte polyploidization and proplatelet formation in low-attachment conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
2759 130 2016 10 PDF Available
Title
Megakaryocyte polyploidization and proplatelet formation in low-attachment conditions
Abstract

•We cultured MKs on tissue-culture treated (TC) and Ultra Low Attachment (ULA) surfaces.•We examined changes in MK polyploidization and proplatelet formation (PPF).•CHRF cells displayed increased polyploidization and arrested PPF on a ULA surface.•ULA culture did not affect primary MK polyploidization, but decreased PPF.•Primary MKs and CHRF cells showed rapid PPF after transfer from a ULA to a TC surface.

In vitro-derived platelets (PLTs), which could provide an alternative source of PLTs for patient transfusions, are formed from polyploid megakaryocytes (MKs) that extend long cytoplasmic projections, termed proplatelets (proPLTs). In this study, we compared polyploidization and proPLT formation (PPF) of MKs cultured on surfaces that either promote or inhibit protein adsorption and subsequent cell adhesion. A megakaryoblastic cell line exhibited increased polyploidization and arrested PPF on a low-attachment surface. Primary human MKs also showed low levels of PPF on the same surface, but no difference in ploidy. Importantly, both cell types exhibited accelerated PPF after transfer to a surface that supports attachment, suggesting that pre-culture on a non-adhesive surface may facilitate synchronization of PPF and PLT generation in culture.

Keywords
Culture-derived platelets; Animal cell culture; Tissue culture; Cell adhesion; Biomedical; Physiology
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Megakaryocyte polyploidization and proplatelet formation in low-attachment conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 111, 15 July 2016, Pages 24–33
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Full-text PDF Download
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