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The mechanism of photoinduced electron transfer in the d-amino acid oxidase–benzoate complex from pig kidney: Electron transfer in the inverted region

Paper ID Volume ID Publish Year Pages File Format Full-Text
27857 44047 2012 12 PDF Available
Title
The mechanism of photoinduced electron transfer in the d-amino acid oxidase–benzoate complex from pig kidney: Electron transfer in the inverted region
Abstract

d-Amino acid oxidase (DAAO) from pig kidney binds flavin adenine dinucleotide (FAD) as a cofactor. The DAAO–benzoate (Bz) complex (DAOB) is remarkably more stable than DAAO. It was previously reported that the fluorescence lifetime of FAD in DAAO is reduced by 66% upon Bz binding. The mechanism of photoinduced electron transfer (ET) in the DAOB monomer from Bz to the excited isoalloxazine (Iso*) was studied by molecular dynamics simulation (MDS) and by Kakitani and Mataga electron transfer theory (KM theory), and compared with that in DAAO. The ET parameters contained in KM theory were determined to obtain the fluorescence lifetime of DAOB (60 ps). The ET mechanism in DAOB is different from other flavoproteins, where neutral tryptophans and tyrosines are the ET donors, since the anionic Bz acts as the anionic ET donor in DAOB. Moreover, ET takes place in the inverted region. Charge transfer interaction (CT) was studied by semi-empirical molecular orbital (MO) methods. The dipole moment in the Iso*–Bz system was lower than that in the isoalloxazine (Iso)–Bz system, which was ascribed to CT from the negatively charged Bz to Iso*. The mean interaction energies between Iso and Bz over 100 snapshots were −16.8 and −8.1 kcal/mol in the excited and the ground states, respectively, with mean charge densities for the excited and the ground states of −0.354 and −0.013 at Iso and −0.646 and −0.987 at Bz, respectively. The ionization potential of Bz was evaluated as 7.3 eV by ET analysis, which was in excellent agreement with that obtained by MO calculations. The dielectric constant between Iso and the donors was 2.2 in DAOB, compared to 5.8 in DAAO. According to the MDS, several water molecules existed around Iso in DAAO that were all removed upon Bz binding resulting in a strongly non-polar environment near Iso in DAOB.

► The mechanism of photoinduced electron transfer (ET) in the DAOB monomer from benzoate (Bz) to the excited isoalloxazine (Iso*) was studied by MD simulation and by Kakitani and Mataga electron transfer theory (KM theory). ► The ET mechanism in DAOB is different from other flavoproteins, where neutral tryptophans and tyrosines are the ET donors, since the anionic Bz acts as the anionic ET donor. ► Charge transfer interaction was also studied by semi-empirical molecular orbital methods. ► The results reveal that ET in DAOB–benzoate complex takes place in the inverted region.

Keywords
Photo-induced electron transfer; Inverted region; d-Amino acid oxidase–benzoate complex; Molecular dynamics simulation; Kakitani and Mataga theory
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The mechanism of photoinduced electron transfer in the d-amino acid oxidase–benzoate complex from pig kidney: Electron transfer in the inverted region
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 250, 15 December 2012, Pages 6–17
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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