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Nanoscopic Characterization of DNA within Hydrophobic Pores: Thermodynamics and Kinetics

Paper ID Volume ID Publish Year Pages File Format Full-Text
2787 135 2015 7 PDF Available
Title
Nanoscopic Characterization of DNA within Hydrophobic Pores: Thermodynamics and Kinetics
Abstract

•dsDNA encapsulated onto hydrophobic SWCNTs is probed regarding its thermodynamics and kinetics.•Encapsulation spontaneity in a 4 nm nanopore (–40 kJ/mol) is annihilated when it narrows down to 3 nm.•Molecular diffusion is anisotropic, starting as Fickian (∼t) and then becomes single-file (∼t1/2).•3D velocity has a distribution maximum at  = 29.9 m/s, twice that for a 3 nucleotide single-strand.•This report is a landmark for the development of next generation in vivo drug delivery technologies.

The energetic and transport properties of a double-stranded DNA dodecamer encapsulated in hydrophobic carbon nanotubes are probed employing two limiting nanotube diameters, D = 4 nm and D = 3 nm, corresponding to (51,0) and (40,0) zig–zag topologies, respectively. It is observed that the thermodynamically spontaneous encapsulation in the 4 nm nanopore (ΔG ≈ –40 kJ/mol) is annihilated when the solid diameter narrows down to 3 nm, and that the confined DNA termini directly contact the hydrophobic walls with no solvent slab in-between. During the initial moments after confinement (t ≤ 2–3 ns), the biomolecule translocates along the nanopore’s inner volume according to Fick’s law (∼t) with a self-diffusion coefficient D = 1.713 × 10−9 m2/s, after which molecular diffusion assumes a single-file type mechanism (∼t1/2). As expected, diffusion is anisotropic, with the pore main axis as the preferred direction, but an in-depth analysis shows that the instantaneous velocity probabilities are essentially identical along the x, y and z directions. The 3D velocity histogram shows a maximum probability located at  = 30.8 m/s, twice the observed velocity for a single-stranded three nucleotide DNA encapsulated in comparable armchair geometries ( = 16.7 m/s, D = 1.36–1.89 nm). Because precise physiological conditions (310 K and [NaCl] = 134 mM) are employed throughout, the present study establishes a landmark for the development of next generation in vivo drug delivery technologies based on carbon nanotubes as encapsulation agents.

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Keywords
DNA; Carbon nanotubes; Thermodynamics; Diffusion; Modelling; Biophysical chemistry
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Nanoscopic Characterization of DNA within Hydrophobic Pores: Thermodynamics and Kinetics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 104, 15 December 2015, Pages 41–47
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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