fulltext.study @t Gmail

Effect of metal cations on the fluorescence lifetimes of pyrene-labeled G-quadruplex probes

Paper ID Volume ID Publish Year Pages File Format Full-Text
27925 44050 2010 7 PDF Available
Title
Effect of metal cations on the fluorescence lifetimes of pyrene-labeled G-quadruplex probes
Abstract

Fluorescence lifetime study of two probes abbreviated as Py-Htelom-Py and Py-TBA-Py, carrying pyrene moieties at both termini and sequences of human telomere and thrombin binding aptamer, respectively are reported. The effect of metal cations (sodium, potassium and strontium) on the photophysical processes was examined in order to elucidate factors that facilitate the production of excimer emission. The fluorescence spectra and emission kinetics data supported the conclusion that pyrene-quadruplex conjugate has multiple conformers in solution and that the relative orientation of pyrene and neighboring nucleobases (guanine, adenine, thymine) play a crucial role in determining both the rate of electron-transfer quenching of pyrene excited state and the efficiency of excimer emission.

Keywords
Excimer; Fluorescence; G-quadruplex; Lifetime; Potassium probe; Pyrene
First Page Preview
Effect of metal cations on the fluorescence lifetimes of pyrene-labeled G-quadruplex probes
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 212, Issue 1, 30 April 2010, Pages 36–42
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering