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Integral production and concentration of surfactin from Bacillus sp. ITP-001 by semi-batch foam fractionation

Paper ID Volume ID Publish Year Pages File Format Full-Text
2793 135 2015 7 PDF Available
Title
Integral production and concentration of surfactin from Bacillus sp. ITP-001 by semi-batch foam fractionation
Abstract

•The used system is a simple method to production and concentration of surfactin.•The surfactin enrichment factor is highly dependent on the agitation and aeration.•The fermented broth has antimicrobial activity against pathogenic microorganisms.

Foam fractionation is an adsorptive separation method that occurs on the liquid-foam interface, when rising bubbles removes active-surface compounds distributed throughout the continuous liquid phase. The formed foam is allowed to build up above the liquid, where, a drainage process results in further enrichment of the surface-active species. In this work, foam fractionation was used the concentration of surfactin from a fermentation broth. The biosurfactant was produced in a culture of Bacillus sp. ITP-001 and the bioreactor was integrated to a fractionation column aiming at the saving of steps in the purification of the product. The production of foam was controlled by fixed aeration and agitation rates. An experimental planning design method of type 22 was used in the experiments set up. The maximum value of surfactin concentration was acquired in the operation with minimum values of aeration and agitation rates. The average surfactin enrichment in these operation conditions reached the value of 28 times and the mass recovery was 94%. The integration of the bioreactor and the separation device was demonstrated as a simple and practical method for surfactin concentration using Bacillus sp. ITP-001, a production method. Assays of antimicrobial activity indicated that the produced surfactin is a powerful antimicrobial agent against several pathogenic microorganisms (Escherichia coli, Candida albicans, Fusarium sp.).

Keywords
Surfactin; Foam fractionation; Bioreactor; Integrated processing; Aeration; Agitation
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Integral production and concentration of surfactin from Bacillus sp. ITP-001 by semi-batch foam fractionation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 104, 15 December 2015, Pages 91–97
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us