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Bioconjugation of peroxidase-like nanostructures with natural enzyme for in-situ amplified conductometric immunoassay of tissue polypeptide antigen in biological fluids

Paper ID Volume ID Publish Year Pages File Format Full-Text
2804 136 2016 8 PDF Available
Title
Bioconjugation of peroxidase-like nanostructures with natural enzyme for in-situ amplified conductometric immunoassay of tissue polypeptide antigen in biological fluids
Abstract

•We design a new conductometric immunoassay of tissue polypeptide antigen.•Prussian blue-gold hybrid nanostructures with peroxidase-like activity were used as the labels.•Natural enzyme and peroxidase-like mimics were utilized for signal amplification.•Nano labels and enzyme labels were aggregated together.

A new conductometric immunoassay based on biofunctionalized prussian blue-gold hybrid nanostructure (PBGN) was designed for sensitive determination of tissue polypeptide antigen (TPA). The immunosensor was prepared by immobilizing anti-TPA capture antibody on the interdigitated transducer, while the PBGN was used for bioconjugation of detection antibody and horseradish peroxidase (HRP). Upon target TPA introduction, the sandwiched immunocomplex was formed between capture antibody and detection antibody on the transducer. Accompanying the PBGN, the carried HRP and prussian blue catalyzed iodine ion into iodine relative to H2O2-KI system, thus resulting in the variations of local conductivity. Under optimal conditions, the immunosensor exhibited good conductometric responses toward target TPA, and allowed the detectable concentration as low as 0.28 pg mL−1. The precision, specificity and reproducibility were acceptable. Importantly, our strategy was also utilized for analysis of 13 human serum specimens, giving well matched results with those obtained from human TPA ELISA kit.

Keywords
TPA, tissue polypeptide antigen; PBGN, prussian blue-gold hybrid nanostructure; HRP, horseradish peroxidase; ELISA, enzyme-linked immunosorbent assay; mAb1, monoclonal rabbit anti-TPA capture antibody; pAb2, polyclonal rabbit anti-TPA detection antibody;
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Bioconjugation of peroxidase-like nanostructures with natural enzyme for in-situ amplified conductometric immunoassay of tissue polypeptide antigen in biological fluids
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 105, Part A, 15 January 2016, Pages 36–43
Authors
,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us