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Efficient acetone–butanol–ethanol (ABE) production by a butanol-tolerant mutant of Clostridium beijerinckii in a fermentation–pervaporation coupled process

Paper ID Volume ID Publish Year Pages File Format Full-Text
2809 136 2016 7 PDF Available
Title
Efficient acetone–butanol–ethanol (ABE) production by a butanol-tolerant mutant of Clostridium beijerinckii in a fermentation–pervaporation coupled process
Abstract

•Mutants with high butanol tolerance were obtained by ARTP mutagenesis.•Enhanced ABE production of 18.8 g/L was achieved during batch fermentation.•Butanol-tolerant mutant was first applied to PV coupled ABE fermentation.•ABE productivity was significantly improved by using BT14 and pervaporation.

Butanol inhibition is one of the major obstacles limiting the economic viability of acetone–butanol–ethanol (ABE) fermentation. In this study, a butanol-tolerant mutant (Clostridium beijerinckii BT14) was generated by atmospheric and room temperature plasmas (ARTP). This mutant showed significant advantage over its parent strain in terms of butanol tolerance. Compared to its parent strain, batch fermentation by this mutant produced 25% higher butanol and 33% higher ABE solvents due to its efficient generation of intracellular NADH and high NADH-dependent butanol dehydrogenase activity. Furthermore, C. beijerinckii BT14 was applied to fed-batch fermentation with pervaporation (PV). As a results, C. beijerinckii BT14 grew to a high cell density and this process generated highly concentrated ABE solution with a high solvent productivity of 0.98 g/(L h) and glucose consumption rate of 2.64 g/(L h). Thus, this work provides an appropriate strategy to develop an efficient process for ABE production in the PV coupled fermentation.

Keywords
Biosynthesis; Butanol tolerance; Atmospheric and room-temperature plasmas; Fed-batch culture; Pervaporation; Process integration
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Efficient acetone–butanol–ethanol (ABE) production by a butanol-tolerant mutant of Clostridium beijerinckii in a fermentation–pervaporation coupled process
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 105, Part A, 15 January 2016, Pages 90–96
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us