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Improved stability and reusability of endoglucanase from Clostridium thermocellum by a biosilica-based auto-encapsulation method

Paper ID Volume ID Publish Year Pages File Format Full-Text
2814 136 2016 6 PDF Available
Title
Improved stability and reusability of endoglucanase from Clostridium thermocellum by a biosilica-based auto-encapsulation method
Abstract

•A novel fusion protein of silica-forming peptide (SFP) and endoglucanase (EG) was prepared.•EG-SEP-encapsulated silica matrix (EG-SFP@Silica) was prepared via SFP-mediated auto-silicification.•EG-SFP@Silica showed higher thermostable activity by 5 °C than free EG-SFP.•EG-SFP@Silica retained 90% of its initial activity with up to 18 uses.

The functional improvement of endoglucanase (EG), a key cellulose-hydrolyzing biocatalyst, is imperative for the practical use of cellulosic materials such as lignocellulose, stove and straws. Here, we employed a bio-inspired silica-encapsulation method to improve the stability and reusability of EG. We introduced a new silica-forming peptide (SFP) from Ectocarpus siliculosus at the C-terminus of EG to generate a recombinant fusion protein, EG-SFP, with auto-silicifying ability. We obtained an EG-SFP-encapsulated silica matrix (EG-SFP@Silica) via the EG-SFP-mediated auto-silicification process under ambient conditions. The immobilization efficiency was 90%. The introduction of SFP did not significantly affect the functionality of EG, and moreover, EG-SFP@Silica demonstrated higher thermostability by 5 °C than free EG-SFP or EG. In addition, EG-SFP@Silica retained 90% of its initial residual activity with up to 18 uses. These results provide a platform for the development of a practical enzymatic hydrolysis process for cellulosic materials.

Keywords
Cellulase; Immobilised enzymes; Immobilization; Polypeptides; Biosilicification; Silica forming peptide
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Improved stability and reusability of endoglucanase from Clostridium thermocellum by a biosilica-based auto-encapsulation method
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 105, Part A, 15 January 2016, Pages 144–149
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us