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Excited state dynamics in the green fluorescent protein

Paper ID Volume ID Publish Year Pages File Format Full-Text
28206 44065 2009 11 PDF Available
Title
Excited state dynamics in the green fluorescent protein
Abstract

The photophysical properties of the wild type green fluorescent protein (wtGFP), the isolated GFP chromophore in solution, and a number of GFP mutants are described. The mechanism of the very efficient radiationless decay of the isolated chromophore is discussed in the light of experimental measurements and theoretical calculations. The dramatic switch in photophysics, from radiationless decay dominated to proton transfer dominated, between the isolated chromophore and wtGFP is illustrated and discussed. The excited state dynamics and their manipulation through mutagenesis are described. The potential for utilizing GFP as a model system for studying the dynamics of proton transfer reactions in proteins, including low barrier hydrogen bonds and proton transport on proton wires, is discussed.

Keywords
Photophysics; Photochemistry; Green fluorescent protein; Proton transfer; Proton wire; Low barrier hydrogen bond; Internal conversion; Isomerization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 205, Issue 1, 10 June 2009, Pages 1–11
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us