fulltext.study @t Gmail

Accelerated vascularization of tissue engineering constructs in vivo by preincubated co-culture of aortic fragments and osteoblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
2824 136 2016 12 PDF Available
Title
Accelerated vascularization of tissue engineering constructs in vivo by preincubated co-culture of aortic fragments and osteoblasts
Abstract

•Preincubated aortic fragments considerably accelerate early angiogenesis.•Co-culture with osteoblasts significantly enhances this angiogenic effect.•A dense microvascular network can be established in vivo using the described method.

There is an urgent critical need for the development of clinically relevant tissue-engineered large bone substitutes that can promote early vascularization after transplantation. To promote rapid blood vessel growth in the engineered tissue, we preincubated aortic fragments, as well as, co-cultures of aortic fragments and osteoblast-like cells in matrigel-filled PLGA scaffolds before implantation into the dorsal skinfold chambers of balb/c mice. Despite an acceptable and low inflammatory response, preincubated aortic fragments accelerate early angiogenesis of tissue-engineered constructs; the angiogenesis was found to occur faster than that observed in previous studies. Thus, the time-period for achieving a denser microvascular network could be reduced to half. In addition, co-culture with osteoblasts enhances this angiogenic effect significantly (against preincubated aortic fragments alone). During the preincubation period, aortic fragments begin to form a network of vessel-like structures additionally supported by osteoblast-like cells. After transplantation, further development of a dense microvasculature continues rapidly. Therefore, preincubation of aortic fragments, especially in co-culture with osteoblast-like cells, in 3D extracellular matrices supports the rapid vascularization of tissue-engineered constructs. This method is a promising approach to establish a dense microvascular network in these constructs.

Keywords
Biodegradation; Tissue cell culture; Animal cell culture; Oxygen transfer; Angiogenesis; Intravital flourescence microscopy
First Page Preview
Accelerated vascularization of tissue engineering constructs in vivo by preincubated co-culture of aortic fragments and osteoblasts
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 105, Part A, 15 January 2016, Pages 230–241
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us