fulltext.study @t Gmail

Extractive bioconversion of poly-ϵ-caprolactone by Burkholderia cepacia lipase in an aqueous two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
2837 137 2015 9 PDF Available
Title
Extractive bioconversion of poly-ϵ-caprolactone by Burkholderia cepacia lipase in an aqueous two-phase system
Abstract

•Optimum condition for extractive bioconversion of PCL in an ATPS was established.•79.8% products and 42.0% lipase were recoverable from separate phases.•The products were characterized qualitatively using GC/MS–MS, TGA, DSC and GPC.•The products consisted of monomeric and dimeric forms of ϵ-caprolactone.

In this study, the optimum condition for extractive bioconversion of poly-ϵ-caprolactone using Burkholderia cepacia lipase in an aqueous two-phase system (ATPS) was established. The optimum condition was found using a combined approach of full factorial design and conventional one-factor-at-a-time studies. Optimum extractive bioconversion was achieved at pH 7.0, 40 °C and composed of 19% (w/w) PEG 3000, 8.1% (w/w) potassium phosphate, with TLL of 28% (w/w) and VR of 80:20. Under this condition, 79.8% products and 42.0% lipase was recovered from the upper and lower phases, respectively. The products consisted of both the monomeric and dimeric forms of ϵ-caprolactone. An inverse relationship was observed between the temperature of bioconversion and the melting, crystallization as well as initial decomposition temperatures of the residual reactant. The molecular weight of the residual reactant decreased with increasing bioconversion temperature whereas the opposite trend was observed for its polydispersity index.

Keywords
ANOVA, analysis of variance; ATPS, aqueous two-phase system; DSC, differential scanning calorimetry; FFD, full factorial design; GC–MS/MS, gas chromatography-tandem mass spectrometry; GPC, gel permeation chromatography; PCL, poly-ϵ-caprolactone; PEG, poly
First Page Preview
Extractive bioconversion of poly-ϵ-caprolactone by Burkholderia cepacia lipase in an aqueous two-phase system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 101, 15 September 2015, Pages 9–17
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us