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UVB/UVC induced processes in model DNA helices studied by time-resolved spectroscopy: Pitfalls and tricks

Paper ID Volume ID Publish Year Pages File Format Full-Text
28431 44075 2006 8 PDF Available
Title
UVB/UVC induced processes in model DNA helices studied by time-resolved spectroscopy: Pitfalls and tricks
Abstract

The investigation of model DNA helices using time-resolved absorption and fluorescence spectroscopy with UVB/UVC excitation knows currently an increasing interest due, in particular, to the use of femtosecond spectroscopy. The study of such complex and fragile systems presents specific difficulties which are not encountered in the experiments performed on the monomeric DNA units. They are related both to the quality of the DNA helices and their sensitivity towards UV radiation. The present paper tackles some of these problems (pitfalls) and describes experimental protocols developed in our laboratory in order to overcome them (tricks). We focus on experiments carried out by fluorescence upconversion spectroscopy, time-correlated single photon counting and nanosecond flash photolysis. We illustrate our experience with examples obtained for double helices containing only adenine–thymine base pairs. We consider this report of pitfalls and tricks, which is far from being complete, as a first step towards a codification of rules for time-resolved studies with model DNA helices. This codification has to be established by common agreement of the various groups working in the field.

Keywords
DNA photodamage; DNA fluorescence; DNA transient absorption; DNA excitons; DNA excited states; DNA energy transfer; Time-resolved spectroscopy; Experimental artifacts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 183, Issues 1–2, 30 September 2006, Pages 1–8
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us