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Synthesis and DNA binding studies by spectroscopic and PARAFAC methods of a ternary copper(II) complex

Paper ID Volume ID Publish Year Pages File Format Full-Text
28464 44076 2006 9 PDF Available
Title
Synthesis and DNA binding studies by spectroscopic and PARAFAC methods of a ternary copper(II) complex
Abstract

A new ternary copper(II) complex, [Cu2(DL-Asp)(phen)3]SO4·4H2O (Phen = 1,10-phenanthroline, DL-Asp = DL-aspartic acid), has been synthesized and characterized by element analysis, infrared spectra and thermal analysis. Spectroscopic studies, including electronic absorption and fluorescence spectra, and parallel factor analysis (PARAFAC) of fluorescence excitation-emission three-way data array were carried out on the DNA binding modes of the complex. The results suggest that at low molar ratio of the complex to DNA (0.25 at most), the end of four-coordinated Cu(II) of the complex binds to DNA by hydrogen bond, which induces the breakage of the secondary structure of DNA. Otherwise, the six-coordinated Cu(II) end of the complex intercalates into the base pair of DNA. Electrostatic effect also takes place between the complex cation and the phosphate group of DNA. Additionally, the equilibrium concentrations of EB-DNA and EB (EB = ethidium bromide) could be directly obtained by PARAFAC algorithm. The results of PARAFAC also indicate that, the conformation of the DNA is changed from B form to BZ mixed form by the interaction with the complex; EB may interact with BZ-DNA by cooperative binding which has a higher affinity than by intercalation with B-DNA. The PARAFAC algorithm is proved a convincing method for studying the interaction of complexes with DNA.

Keywords
Ternary copper(II) complex; Synthesis; DNA binding; PARAFAC algorithm
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Synthesis and DNA binding studies by spectroscopic and PARAFAC methods of a ternary copper(II) complex
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 184, Issue 3, 15 December 2006, Pages 241–249
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us