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Spectroscopic investigation of flavoproteins: Mechanistic differences between (electro)chemical and photochemical reduction and oxidation

Paper ID Volume ID Publish Year Pages File Format Full-Text
28486 44077 2008 5 PDF Available
Title
Spectroscopic investigation of flavoproteins: Mechanistic differences between (electro)chemical and photochemical reduction and oxidation
Abstract

The spectroelectrochemical investigation of proteins is an important method to study their redox properties and to elucidate protein ET pathways. Such studies are often more complicated for proteins than for small molecules because the redox-active cofactor is not easily accessible and/or the proteins are quite sensitive. For the determination of redox potentials it is necessary to work under equilibrium conditions which can be achieved by the presence of redox mediators. The proteins can be reduced and oxidized by chemical, electrochemical, and photochemical methods, each of which are briefly discussed. Differences in the mechanism of chemical and photochemical reduction of flavoproteins are pointed out. Photochemical reduction by irradiation with blue-light in the presence of EDTA can lead to the formation of flavoprotein semiquinone radicals which otherwise could not be observed.

Keywords
Spectroelectrochemistry; Protein electron transfer; Photochemical reduction; Flavins and flavoproteins; LOV domains
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 200, Issue 1, 15 November 2008, Pages 34–38
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us