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Functionalised, photostable, fluorescent polystyrene nanoparticles of narrow size-distribution

Paper ID Volume ID Publish Year Pages File Format Full-Text
28506 44078 2012 8 PDF Available
Title
Functionalised, photostable, fluorescent polystyrene nanoparticles of narrow size-distribution
Abstract

Fluorescent nanoparticles continue to be of wide interest, as they have many advantages over single fluorescent molecules for biological imaging and sensing applications, such as increased fluorescence intensity and reduced photobleaching. In the following work, styrene was copolymerised with a newly synthesised, fluorescein-based, vinylic crosslinking monomer, by emulsion polymerisation, to create a series of different sized fluorescent nanoparticles (35–100 nm), each of narrow size-distribution. The particles were found to be highly fluorescent and with lower photobleaching compared to fluorescein isothiocyanate (FITC), offering an attractive alternative. The fluorescence excitation and emission spectra were recorded, being similar to fluorescein, but with interesting variation in the excitation spectra. The particles also have a wide range of potential uses, such as examining particle uptake activity of a macrophage cell line, also demonstrated. The nanoparticles were coated with albumin to provide functionality for potential conjugation to biological targeting agents.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Fluorescent nanoparticles are advantageous over commonly used organic fluorophores such as increased fluorescence and reduced photobleaching. ► Polystyrene was copolymerised with a newly synthesised fluorescent vinylic crosslinking monomer. ► Highly fluorescent and photostable nanoparticles of various sizes were obtained. ► Particles were functionalised by an albumin coating for potential conjugation of a bioactive reagent.

Keywords
Fluorescent nanoparticles; Polystyrene nanoparticles; Fluorescein; Emulsion polymerisation
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Functionalised, photostable, fluorescent polystyrene nanoparticles of narrow size-distribution
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 228, Issue 1, 15 January 2012, Pages 60–67
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us