fulltext.study @t Gmail

Esterification of glycerol and ibuprofen in solventless media catalyzed by free CALB: Kinetic modelling

Paper ID Volume ID Publish Year Pages File Format Full-Text
2859 137 2015 9 PDF Available
Title
Esterification of glycerol and ibuprofen in solventless media catalyzed by free CALB: Kinetic modelling
Abstract

•Ibuprofen solubility in glycerol grows exponentially with temperature.•Stable nanoemulsions glycerol–ibuprofen are obtained.•Activity is a hyperbolic function of stirrer speed, water content and enzyme and ibuprofen concentrations.•Kinetic modelling suggests that ibuprofen reacts in the glycerol phase.•High thermal stability exists till 80 °C, probably due to interfacial enzyme immobilization.

The enzymatic reaction of ibuprofen and glycerol in solventless media without and with the optimum content in water to obtain ibuprofen monoester is studied, selecting an adequate kinetic model for both cases. Preliminary runs led to fixing concentration of lipase CALB-L at 2 g L−1 and stirring speed at 720 rpm, and showed that a 10% water content enhances both initial rate of reaction and final yield to the monoester. For further comparison of hydrated and non-hydrated systems, kinetic runs were performed at several ibuprofen initial concentrations (20–100 g L−1) and temperatures (50–80 °C). In all cases, the formation of an emulsion was observed and analyzed by focused beam reflectance measurement (FBRM), dynamic light scattering (DLS) and turbidimetry. Considering kinetic and thermodynamical data, several phenomenological kinetic models were proposed and fitted to all the available data, using physical and statistical criteria to select the best ones. In both cases, the chosen model was a reversible Michaelis–Menten model of pseudo-first order with respect to the concentration of ibuprofen with no inhibition caused either by substrates or products. Kinetic constants showed that water addition resulted in mass transfer enhancement.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Lipase; Microemulsion; Water content; Kinetic model; Ibuprofen; Glycerol
First Page Preview
Esterification of glycerol and ibuprofen in solventless media catalyzed by free CALB: Kinetic modelling
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 101, 15 September 2015, Pages 228–236
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us