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Highly efficient fluorescent label unquenched by protein interaction to probe the avidin rotational motion

Paper ID Volume ID Publish Year Pages File Format Full-Text
28596 44081 2006 8 PDF Available
Title
Highly efficient fluorescent label unquenched by protein interaction to probe the avidin rotational motion
Abstract

Fluorescent dye alexa-fluor-594 was fixed to biotin with a spacer chain of four atoms. The static, dynamic fluorescence and fluorescence polarization properties of the avidin/labeled-biotin conjugate were investigated and compared with those of free fluorescent dye and directly labeled avidin. With 0.8 label on the average per avidin, one single fluorescence lifetime and one single correlation time related to the actual protein motion were recorded. Moreover, the excited state fluorescent probe is stabilized in the conjugate, with a quantum yield of 0.87, by slowing down the non-radiative processes, while fluorescence lifetime and fluorescence quantum yield increase by 10%, with respect to free alexa-fluor-594.

Keywords
Fluorescent label; Avidin; Alexa-fluor-594; Avidin alexa fluor conjugate; Biotin
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Highly efficient fluorescent label unquenched by protein interaction to probe the avidin rotational motion
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 184, Issues 1–2, 15 November 2006, Pages 204–211
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us