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Fluorescence properties of aza-helicenium derivatives for cell imaging

Paper ID Volume ID Publish Year Pages File Format Full-Text
28638 44083 2011 7 PDF Available
Title
Fluorescence properties of aza-helicenium derivatives for cell imaging
Abstract

In this work the optical properties of three N-methyl-aza-[5]-helicenium salts having the same organic moiety but different counterions (I−, NO3− and COOCF3−) were investigated in different media. In particular, the absorption and fluorescence spectra of the compounds were strongly affected by the solvent properties. Fluorescence synchronous scans and time resolved fluorescence measurements indicated that in alcoholic solvents a dual emission behaviour occurred, likely due to aggregation phenomena which can be tuned by the counterion nature. The emission properties of the compounds were tested for cell labelling aims using Hude cells. The occurrence of aggregation processes controlled by the media properties resulted in a dual colour labelling in different cell compartments. In particular, the cell interior was visualized as a green fluorescence corresponding to a higher energy emission assigned to the dye molecules in monomeric form, while longer wavelength fluorescence was observed in cytoplasm, likely due to aggregation of the dye in this environment.

► In this work the fluorescence properties of helicenium salts having different counterions (I−, NO3− and COOCF3−) were investigated in different solvents. ► A dual emission behaviour was observed in alcoholic solvents, likely due to formation of aggreagate species in the ground state. ► The fluorescence properties of the compounds were tested for cell labelling aims using Hude cells. ► The dual emission behaviour controlled by the medium properties resulted in a dual colour labelling in different cell compartments.

Keywords
Dyes; Fluorescence; Solvent effect; Imaging; Cell labelling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 222, Issues 2–3, 15 August 2011, Pages 307–313
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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