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Process optimization of lipase catalyzed synthesis of diesters in a packed bed reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
2867 140 2015 4 PDF Available
Title
Process optimization of lipase catalyzed synthesis of diesters in a packed bed reactor
Abstract

•Lipase catalysed synthesis of wax esters like dibehenyl adipate and dibehenyl sebacate were investigated.•Enzyme NS 435 was found to be the most effective biocatalyst.•75% of adipic acid and 83% of sebacic acid were converted to their behenyl esters.•Maximum amount of diesters were produced.•89% of dibehenyl adipate and 91% of dibehenyl sebacate were isolated from the packed bed reactor.

Wax esters are fine chemicals which are produced in low volume but are highly priced with varied industrial applications. They are formulated in numerous personal care products due to their excellent emollient properties. The conventional technology for the preparation of wax esters are based on reaction between long chain carboxylic acids and alcohols at high temperatures in presence of a strong catalyst. This process is unselective and the removal of used catalyst and byproducts requires several stages which increases the production cost. Enzyme technology is an alternative which is eco friendly and requires lesser stages of purification. The present study aims at the enzymatic preparation of wax esters like dibehenyl adipate and dibehenyl sebacate in presence of immobilized lipases from different microbial origin e.g., RM-IM, TL IM, NS40013 and NS 435. Various reaction parameters like temperature, reaction time, mole ratio, enzyme concentration and solvent selection were optimized. The work clearly indicates NS 435 as the most efficient enzyme and can be recycled for 20 times without significant change in product quality. Trial run was given in a packed bed bioreactor using NS435 which produced 89% and 91% of dibehenyl adipate and dibehenyl sebacate, respectively.

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Keywords
Lipase; Wax esters; Immobilized enzymes; Dibehenyl adipate; Dibehenyl sebacate; Packed bed Bioreactor; Optimisation
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Process optimization of lipase catalyzed synthesis of diesters in a packed bed reactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 102, 15 October 2015, Pages 2–5
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us