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DNA detection via programmed core-shell nanodot-assembly with concomitant fluorescence modulation

Paper ID Volume ID Publish Year Pages File Format Full-Text
28720 44087 2008 5 PDF Available
Title
DNA detection via programmed core-shell nanodot-assembly with concomitant fluorescence modulation
Abstract

In this work, we designed and demonstrated a new DNA probe working on the principle of the fluorescence change with improved detection capability via building a cruciform DNA hybrid with target ODNs. In our oligodeoxynucleotide-pyrene-oligodeoxynucleotide (OPO) probe, hybridisations between the probes (OPO) and target oligodeoxynucleotides (ODNs) are programmed to induce the formation of core-shell (pyrene-ODN) nanodot structure in aqueous solution to generate and maintain their excimer emission more efficiently. Since this novel OPO probe showed wide detection range, high-excimer emission efficiency and high reliability of excimer emission intensity, the probe is capable of application to diagnosis of genetic disease and detection of increased DNA copy number and occurrence of mutation in a real-time PCR.

Keywords
DNA nanostructure; Excimer emission; Fluorescent probes; Real-time PCR; Nanobiotechnology
First Page Preview
DNA detection via programmed core-shell nanodot-assembly with concomitant fluorescence modulation
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 196, Issue 1, 30 April 2008, Pages 94–98
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering