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A comparative study on the physico-chemical properties of bacterial capsular polysaccharides from different serotypes of Klebsiella

Paper ID Volume ID Publish Year Pages File Format Full-Text
28788 44091 2006 8 PDF Available
Title
A comparative study on the physico-chemical properties of bacterial capsular polysaccharides from different serotypes of Klebsiella
Abstract

Comparative studies on various physico-chemical properties of bacterial capsular polysaccharides from different serotypes of Klebsiella have been investigated. A correlation of the primary structures with the solution properties of different polymers has been established based on spectrophotometric, spectrofluorometric and viscometric measurements. Absorption studies on four experimental biopolymers (K7, K14, K17 and K26) with four cationic dyes, pinacyanol choloride (PCYN), acridine orange (AO), toluidine blue (TB) and phenosafranin (PSF) indicated that chromotropic capability of the polymers with respect to induction of metachromasy followed the order: K7 > K14 > K26 > K17. Formation of (1:1) stoichiometric polymer–dye complexes suggested stacking conformation in all the four polymers. Fluorescence quenching of AO and PSF dye induced by the polymers also revealed the quenching efficiency of polymers to follow the same order as revealed in the absorption studies. The Stern–Volmer constant (KSV) also supported similar trend. The order of chromotropic character and fluorescence quenching of the polymer were in conformity with their charge densities. Viscosity measurements of all the four capsular polysaccharide solutions showed the characteristic behavior of polyelectrolytes indicating sharp increase in reduced viscosity of the aqueous polysaccharide solution at lower dilution. Molecular weight of the bacterial polysaccharides were also determined from viscometric measurements.

Keywords
Capsular polysaccharides; Dye–polymer interaction; Metachromasia; Viscosity; Fluorescence quenching
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A comparative study on the physico-chemical properties of bacterial capsular polysaccharides from different serotypes of Klebsiella
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 178, Issue 1, 20 February 2006, Pages 98–105
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us