fulltext.study @t Gmail

Tuning the DNA binding modes of an anthracene derivative with salt

Paper ID Volume ID Publish Year Pages File Format Full-Text
28838 44096 2006 12 PDF Available
Title
Tuning the DNA binding modes of an anthracene derivative with salt
Abstract

The DNA binding properties of an anthracene derivative with substituents at the 9 and 10 positions, carrying four positive charges, are examined in calorimetric, spectroscopic and photocleavage studies. Isothermal titration calorimetric data indicated exothermic binding of the ligand to calf thymus DNA with a binding constant of (1.4 ± 0.5) × 105 M−1 and this value is much greater than binding of similar monocationic derivatives. The values for the other binding parameters were, ΔH = −3.5 ± 0.4 kcal/mol; ΔS = 11.6 ± 1.6 cal/mol K, and a binding site size of ∼4 base pairs. Absorption spectral studies indicated small, but significant red shifts in the vibronic bands, and ∼70% of hypochromism. The binding plots indicated bi-phasic binding of the ligand. At higher ionic strengths, the red shifts in the absorption spectra were abolished but significant hypochromism persisted.Excitation and sensitized fluorescence spectral studies indicated weak energy transfer from the DNA bases to the ligand. Further more, energy transfer was reduced substantially at higher ionic strengths. Strong induced circular dichroism bands are noted, in the 300–400 nm region, and these are most likely dominated by the contributions from the groove bound form as well as the intercalated chromophore. Helix melting studies indicated improvement in the helix stability, and substantial increase in the melting temperature (ΔTm > 17 °C). Differential scanning calorimetric data, on the other hand, indicated only minor improvements in the thermodynamic parameters. Irradiation of a mixture of the ligand (2 μM) and supercoiled pUC18 DNA (20 μM, @374 nm) resulted in the efficient formation of nicked circular DNA (>90%) in an hour. The data indicated at least two distinct binding modes, and one of these persisted at high ionic strengths (375 mM NaCl). Substitution at 9 and 10 positions of the anthracene ring system with positively charged residues resulted in multiple binding modes, and these are resolvable in ionic strength studies.

Keywords
Isothermal titration calorimetry; DSC; Photocleavage; DNA; Anthracene derivatives
First Page Preview
Tuning the DNA binding modes of an anthracene derivative with salt
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 177, Issue 1, 1 January 2006, Pages 43–54
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us