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Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection

Paper ID Volume ID Publish Year Pages File Format Full-Text
2905 142 2015 9 PDF Available
Title
Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection
Abstract

•Dual-functional PA-66 nanofibrous membrane was prepared for OPs protection.•The membrane has particle filtration efficiency over 99%.•OPH immobilized on PA-66 nanofibers catalyzed OPs degradation.•Degradation efficiency for methyl parathion was about 40%.•The dual-functional membrane has good stability.

In both civilian and military domains, the increasing use of highly toxic organophosphates (OPs) has created an urgent demand for developing an effective method that could protect the human body from OP poisoning. In this study, a novel dual-functional protecting material with both OP filtration and degradation functions was designed and fabricated. The filtration function was endowed by the electrospinning polyamide 66 (Nylon 66, PA-66) nanofibrous membrane, while the OP degradation function was based on the immobilization of organophosphorus hydrolase (OPH) on membrane through glutaraldehyde (GA) crosslinking. A systematic study of the relationship between the membrane structure, filtration ability and OP degradation activity revealed that PA-66 nanofibrous enzyme with 20 μm thickness exhibited perfect uniform morphology, and the particle filtration efficiency was over 99%. The nanofibrous enzyme exhibited a specific activity of 41 U/g; the enzyme activity recovery was 53.3%, and the methyl parathion (MP) degradation efficiency was 40%. Moreover, the nanofibrous enzyme expressed excellent organic solvent stability; approximately 70% of its initial activity was retained after incubation in xylene solvent for 24 h at 25 °C. The residual activity was 40% after being stored for 30 d at 25 °C. After 10 repeated uses, the residual activity of the nanofibrous membrane was 37%. This study demonstrated the application potential of dual-functional PA-66 nanofibrous enzyme in biochemical protection, aimed at various military and civilian applications.

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Keywords
Nanofibrous membrane; Immobilized enzymes; Biodegradation; Particle filtration; Enzyme biocatalysis; Kinetic parameters
First Page Preview
Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 98, 15 June 2015, Pages 47–55
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering