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Facile self-assembly of magnetite nanoparticles on three-dimensional graphene oxide–chitosan composite for lipase immobilization

Paper ID Volume ID Publish Year Pages File Format Full-Text
2908 142 2015 9 PDF Available
Title
Facile self-assembly of magnetite nanoparticles on three-dimensional graphene oxide–chitosan composite for lipase immobilization
Abstract

•Amine-functionalized Fe3O4 nanoparticles were self-assembly on a 3D GO–CS composite.•The composite, thus, showed high magnetization.•The composite exhibited large surface area, abound functional groups.•The composite is utilized to immobilize lipase via different routes.

Three dimensional (3D) magnetic graphene oxide–chitosan (GO–CS) composites with orderly self-assembled magnetite (Fe3O4) nanoparticles (GO–CS–Fe3O4) were successfully fabricated. First, the covalent functionalization of graphene oxide (GO) using chitosan (CS) was successfully accomplished via a facile amidation process, forming a 3D network. In the as-fabricated GO–CS composites, GO sheets remained less aggregated, exhibiting a large surface area, and the interconnected pores in the hydrogels allowed object molecules to diffuse easily into the composites. Subsequently, the GO–CS–Fe3O4 composites were synthesized using FeCl3·6H2O and GO–CS composite as the starting materials through a solvothermal reaction. The resulting composite combined the features of the high surface area of GO, abundant amino, and hydroxyl functional groups of CS, and the magnetic response of magnetic nanoparticles. For further application, these composites were utilized to immobilize Candida rugosa lipase via different routes, and they showed excellent immobilization capacities.

Keywords
Graphene oxide–chitosan; Fe3O4 nanoparticles; Immobilised enzymes; Biocatalysis; Lipase; Enzyme activity
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Facile self-assembly of magnetite nanoparticles on three-dimensional graphene oxide–chitosan composite for lipase immobilization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 98, 15 June 2015, Pages 75–83
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us